Monoclonal antibodies directed against surface molecules of multicell spheroids
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Monoclonal antibodies directed against surface molecules of multicell spheroids semi-annual status report, report period, 01-01-94 - 06-01-94 by

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Published by National Aeronautics and Space Administration, National Technical Information Service, distributor in [Washington, DC, Springfield, Va .
Written in English

Subjects:

  • Antibodies.,
  • Cells (Biology),
  • Gravitational physiology.,
  • Microgravity.,
  • Tissues (Biology)

Book details:

Edition Notes

Statementprincipal investigator, Andrew O. Martinez.
SeriesNASA contractor report -- NASA CR-196385.
ContributionsUnited States. National Aeronautics and Space Administration.
The Physical Object
FormatMicroform
Pagination1 v.
ID Numbers
Open LibraryOL15404204M

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Monoclonal antibody, antibody produced artificially through genetic engineering and related techniques. Production of monoclonal antibodies was one of the most important techniques of biotechnology to emerge during the last quarter of the 20th century. In vitro multicellular cancer spheroids have begun to bridge the complexity gap between monolayer cell culture and in vivo tumors and have become valuable models in the study of drug resistance. Spheroids exhibit many features of the tumor microenvironment and model the avascular region of tumors that is dependent on diffusion (Fig. (Fig.1 1) by: Generation of monoclonal antibodies against the CTOS surface. To generate antibodies that recognize molecules on the CTOS surface, we intraperitoneally injected colorectal CTOSs, C45, into a mouse without adjuvant, expecting to preserve the 3D by: 5. Production and use of monoclonal antibodies for the characterization of avian reoviruses and Nelson Bay Guidelines on monoclonal antibody production / National Health and Medical Research Council; Monoclonal antibodies directed against surface molecules of multicell spheroids [microform] / principal.

Unlike previously reported monoclonal antibodies to tumor cell-surface antigens, TNT-1 showed (1) a linear uptake that increased over time without saturation in tumor spheroids and (2) an unexpected uptake by a subpopulation of cells in the viable outer rim of the spheroids. Monoclonal T-cell Receptors (TCRs) Antibodies can bind to molecules expressed at the surface of target cells (as well as to soluble molecules) but are not effective against the peptide fragments that antigen-presenting cells contain tucked within their histocompatibility molecules. T-cell receptors are the ligands needed for that job.   Background: Multicell spheroids from the human osteosarcoma cell line, OHS, were incubated with increasing concentrations of the monoclonal antibodies TP-1, TP-3 and having different affinities (K(a) = 10 8 M 1 10 9 M 1 and 10 11 M 1, respectively).Materials and Methods: Uptake and penetration of the fluorescein labelled antibodies were studied using confocal Cited by: 7. This successful laboratory handbook focuses on procedures for hybridoma generation and gives detailed test protocols for the application of monoclonal antibodies. Immunization, cell preparation, hybridization, cell culture and cloning of hybridomas, prevention of back-mutation, as well as purifi- cation, labeling, and characterization of the antibodies ob- tained, are only a few keywords.

Human cell differentiation molecules, also known as "CD", are cell-surface molecules reacting with different monoclonal antibodies. The fraction of cells belonging to each immunophenotype is. Monoclonal Antibodies to Human Cell Surface Antigens. Alice Beare. This is followed by a table that lists all human molecules with approved CD names, tabulating alternative names, key structural features, cellular expression, major known functions, and usefulness of the molecules or antibodies against them in research or clinical Cited by: Monoclonal Antibodies are cells derived by cell division from a single ancestral onals are a class of antibodies with identical offspring of a hybridoma and are very specific for a particular location in the body derived from a single clone and can be grown indefinitely. Monoclonal Antibodies recognize and bind to antigens in order to discriminate between specific epitopes which. Establishment of monoclonal antibodies against a novel eosinophil-specific cell surface molecule, major facilitator super family domain containing 10 Author links open overlay panel Yuji Motoi a 1 Mayumi Saeki a 1 Tomoe Nishimura a Kazufumi Katayama a Noriko Kitamura a Hitoshi Ichikawa b Hiroyuki Miyoshi c Osamu Kaminuma a Takachika Hiroi aCited by: 2.